Webb12 jan. 2024 · ATAC-pipe是中科大Kun Qu实验室开发的一个打包软件包,用于分析bulk ATAC-Seq。 主要功能包括: 鉴定显著差异的开放染色质区域 motif分析及相关的TFs对转录调控的影响 TF和motif的足迹分析以及核小体占位的可视化 基于相关性的细胞聚类分析 构建TF调控网络,评估TFs和基因间的相互作用 详细用法参考: … HINT-ATAC is part of the Regulatory Genomics Toolbox, and some of its modules need to be locally installed before running the pipeline. Here is the configuration page. Be extra thorough in this step because a lot of subtle things can be missed in this step, which will affect downstream work. Consult … Visa mer Find transcription factor footprints and test for differential chromatin openness in TF motifs using ATAC-Seq. This method is based on the original HINT-ATAC footprint method by Zhijian Li et al. Visa mer You can run the pipeline using an interactive node like this: This is sufficient for small jobs or running small parts of the pipeline, but not … Visa mer To answer the questions: 1. Which TF binding sites have the most different chromatin openness states between two conditions? 2. At … Visa mer This is only applicable if compute_factors function from RGT HINT does not have a pseudo-signal adjustment. Read on if you are confused. In the differential analysis process, reads are … Visa mer
Fully-automated and ultra-fast cell-type identification using …
Webb20 apr. 2024 · With Hint-ATAC [ 31 ], scATAC-pro enables footprinting analysis of either one cell cluster or differential TF binding between two groups of cell clusters. Integration of multiple scCAS data sets To integrate multiple scCAS data sets, assuming each data set is processed by scATAC-pro, we first merge peaks identified in each data set. WebbThis library has been used for implementation of several tools as ChIP-Seq differential peak callers ( THOR ), ATAC/DNase-seq footprinting method ( HINT ), motif bindng sites analysis ( Motif analysis) and the visualization tool RGT-Viz. Figure 1 shows a typical pipeline for analysis of histone modifications. bonsai houston
Identification of Transcription Factor Binding Sites using ATAC …
WebbExecution. 1. Change working directory & activate conda environment. Execute always from within top level of the pipeline directory (ie atacseq_pipeline/). Snakemake commands only work from within the snakemake conda environment. cd atacseq_pipeline conda activate snakemake. 2. WebbRGT is an open source Python 3.6+ library for analysis of regulatory genomics. RGT is programmed in an oriented object fashion and its core classes provide functionality for … Webb5 jan. 2024 · HINT-ATAC文章代码复现 - 蒟蒻、 - 博客园. 你好!. 欢迎来到本站!. 这里是我的个人博客,主要存放我的学习记录、生活随笔和其他的一些乱七八糟的东西。. goddess with sword